Effect of Total Flavones of Astragalus on Apoptosis and Inflammation of Alveolar Epithelial Cells Induced by Tumor Necrosis Factor Alpha through microRNA-221

To explore the effect and molecular mechanism of total flavonoids Astragalus on apoptosis inflammation alveolar epithelial cells induced by tumor necrosis factor alpha. Human were divided into control group, alpha alpha+trifluoroacetic acid-low, acid-medium, acid-high alpha+anti-microRNA-NC alpha+microRNA-221 inhibitor acid-high+microRNA-NC acid-H+microRNA-221 mimic group. Real-time fluorescence quantitative polymerase chain reaction was used to detect expression microRNa-221; (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide cell proliferation inhibition rate; flow cytometry cells; enzyme-linked immunosorbent assay levels interleukin-1 beta, I interleukin-6 interleukin-10. In alpha-induced cells, microRNA-221 increased, rate increased level interleukin-10 decreased (p<0.05). After treatment with medium high concentrations flavones Astragalus, in decreased, beta Inhibition can inhibit cells. Overexpression reverse Total inhibited down-regulating microRNA-221.